浙江大學(xué)生命科學(xué)學(xué)院生物科學(xué)系導(dǎo)師：應(yīng)盛華 正文

　　姓名：應(yīng)盛華
　　性別：男
　　所在學(xué)院：生命科學(xué)學(xué)院
　　職稱(chēng)：副教授

　　工作研究領(lǐng)域：
　　主要從事真菌基因組學(xué)、真菌分子生物學(xué)及生防真菌研究與開(kāi)發(fā)。
 
　　個(gè)人簡(jiǎn)介：
　　應(yīng)盛華，男，1973年9月生，博士，浙江大學(xué)微生物研究所副教授、碩導(dǎo)。
　　1995年畢業(yè)于山東大學(xué)微生物系獲學(xué)士學(xué)位，并分別于2000、2006年獲浙江大學(xué)微生物學(xué)碩士和博士學(xué)位。
　　本人長(zhǎng)期從事真菌抗逆、生防真菌侵染的功能基因以及遺傳改造工作，目前正在積極開(kāi)展真菌全基因組測(cè)序和功能基因組學(xué)的研究。
　　目前，本人已主持國(guó)家自然科學(xué)基金三項(xiàng)、“863”子課題一項(xiàng)、省部級(jí)重點(diǎn)項(xiàng)目一項(xiàng)和國(guó)家自然科學(xué)基金重點(diǎn)項(xiàng)目子課題一項(xiàng)，以及作為骨干成員參加“973”項(xiàng)目、國(guó)際合作項(xiàng)目各一項(xiàng)。
　　本人入選浙江大學(xué)青年骨干教師培養(yǎng)計(jì)劃、榮獲2006年度浙江大學(xué)校級(jí)獎(jiǎng)教金、2007年度浙江大學(xué)優(yōu)秀班主任稱(chēng)號(hào)、2007和2008年度浙江大學(xué)生科院先進(jìn)工作者稱(chēng)號(hào)、2009年校百篇優(yōu)秀畢業(yè)論文（本科畢設(shè)）指導(dǎo)教師獎(jiǎng)。

　　主持項(xiàng)目：
　　1． 國(guó)家自然科學(xué)基金項(xiàng)目(30971960)球孢白僵菌耐熱力與多菌靈抗性負(fù)關(guān)聯(lián)的生理和分子機(jī)制 2010．01-2012．12 經(jīng)費(fèi)：32萬(wàn)
　　2． 國(guó)家自然科學(xué)基金項(xiàng)目(30600410)轉(zhuǎn)蘇云金芽孢桿菌營(yíng)養(yǎng)期毒蛋白Vip3A的球孢白僵菌菌株及其侵染生物學(xué)2007．01-2009．12 經(jīng)費(fèi)：22 萬(wàn)
　　3． 浙江省重大科技專(zhuān)項(xiàng)（優(yōu)先主題）(2008C02007-1)蔬菜和茶樹(shù)重大害蟲(chóng)生防菌的創(chuàng)制和產(chǎn)業(yè)化研究 2008．07-2010．07 經(jīng)費(fèi)：100 萬(wàn)
　　4． 國(guó)家自然科學(xué)基金重點(diǎn)項(xiàng)目子課題 生防真菌適應(yīng)季節(jié)性逆境的分子生態(tài)機(jī)制研究 2010．01-2013．12 經(jīng)費(fèi)：60萬(wàn)
　　5． “863”計(jì)劃（子項(xiàng)目）(2007AA021305)核輻射環(huán)境微生物資源的開(kāi)發(fā)利用 2007．06-2010．12 經(jīng)費(fèi)：37萬(wàn)
　　6． 浙江省教育廳基金 (G20020247)球孢白僵菌孢內(nèi)活力相關(guān)多糖組分的研究 2002．7-2004．7 經(jīng)費(fèi)：1．5萬(wàn)
　　7． 浙江出入境檢驗(yàn)檢疫局 （浙大05-308104-4）產(chǎn)黃曲霉毒素真菌PCR檢測(cè)技術(shù)的研究 2005．1-2007．12 經(jīng)費(fèi)：6．0萬(wàn)

　　主參項(xiàng)目：
　　1． “973”項(xiàng)目 害蟲(chóng)生防真菌致病、抗逆的分子機(jī)理與遺傳改良 (2009CB118904) (2/8) 2009．01-2013．12 經(jīng)費(fèi)：450萬(wàn)
　　2． “863”項(xiàng)目 細(xì)菌、真菌類(lèi)生物殺蟲(chóng)劑研究和創(chuàng)制 (2006AA10A212) (2/4) 2006．12-2010．10經(jīng)費(fèi)：54萬(wàn)
　　3． 科技部國(guó)際合作項(xiàng)目 (2007DFA31100) 中方第二負(fù)責(zé)人 2008．01-2010．12耐夏季高溫和紫外輻射的高效殺蟲(chóng)劑研制 經(jīng)費(fèi)：160萬(wàn)

　　發(fā)表論文：
　　1． Gao Q, Jin K, Ying SH (equally first author), Zhang Y, Xiao G, et al． Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M． acridum． PLoS Genetics, 2011, 7: e1001264．
　　2． Qin Y, Ying SH (Co-corresponding), Chen Y, Shen ZC, and Feng MG． Integration of Insecticidal Protein Vip3Aa1 into Beauveria bassiana Enhances Fungal Virulence to Spodoptera litura Larvae by Cuticle and Per Os Infection． Applied and Environmental Microbiology, 2010, 76: 4611-4618．
　　3． Ying SH and Feng MG． A conidial protein (CP15) of Beauveria bassiana contributes to the conidial tolerance of the entomopathogenic fungus to thermal and oxidative stresses． Applied Microbiology and Biotechnology, 2011, 90:1711–1720．
　　4． Ying SH, Wang XH and Feng MG． Characterization of a thioredoxin (BbTrx) from the entomopathogenic fungus Beauveria bassiana and its expression in response to thermal stress． Canadian Journal of Microbiology, 2010, 56:1-9．
　　5． Ying SH and Feng MG． Means to mediating accumulation of hydrophobin-like proteins on the wall of Beauveria bassiana conidia for improved tolerance to thermal stress． Journal of General and Applied Microbiology, 2007, 53: 309-314．
　　6． Ying SH and Feng MG． Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana． Applied Microbiology and Biotechnology, 2006, 72: 206-210．
　　7． Ying SH． and Feng MG． Medium components and culture conditions affect the thermotolerance of aerial conidia of fungal biocontrol agent Beauveria bassiana． Letters in Applied Microbiology, 2006, 43: 331–335．
　　8． Ying SH and Feng MG． Relationship between thermotolerance and hydrophobin-like proteins in aerial conidia of Beauveria bassiana and Paecilomyces fumosoroseus as fungal biocontrol agents． Journal of Applied Microbiology, 2004, 97: 323-331．
　　9． Wang ZL, Ying SH, Feng MG． Gene cloning and catalysis features of a new mannitol-1-phosphate dehydrogenase (BbMPD) from Beauveria bassiana． Carbohydrate Research, 2010, 345: 50-54．
　　10． Xie XQ, Ying SH, Feng MG． Characterization of a new Cu/Zn-superoxide dismutase from Beauveria bassiana and two site-directed mutations crucial to its antioxidation activity without chaperon． Enzyme and Microbial Technology, 2010, 46: 217-222．
　　11． Li J, Ying SH, Shan LT, Feng MG． A new non-hydrophobic cell wall protein (CWP13) of Metarhizium anisopliae increases conidial hydrophobicity when expressed in Beauveria bassiana． Applied Microbiology and Biotechnology, 2010, 85: 975-984．
　　12． Yao SL, Ying SH, Feng MG, Hatting JL． In vitro and in vivo responses of fungal biocontrol agents to the gradient doses of UV-B and UV-A irradiations． BioControl 2010, 55: 413-422．
　　13． Liu Q, Ying SH, Feng MG and Jiang XH． Physiological implication of intracellular trehalose and mannitol changes in response of entomopathogenic fungus Beauveria bassiana to thermal stress． Antonie van Leeuwenhoek International Journal of General and Molecular Micriobiology, 2009, 95: 65-75． ．
　　14． Ye SD, Ying SH, Chen C and Feng MG． New solid-state fermentation chamber fits up bulk production of aerial conidia of fungal biocontrol agents on rice． Biotechnology Letters, 2006, 28: 799-804．
　　15． Zou G, Ying SH, Sheng ZC and Feng MG． Multi-sited mutations of beta-tubulin are involved in benzimidazole resistance and thermotolerance of fungal biocontrol agent Beauveria bassiana． Environmental Microbiology, 2006, 8: 2095-2106．
　　16． Jiang Q, Ying SH and Feng MG． Enhanced frequency of Beauveria bassiana blastospore transformation by restriction enzyme-mediated integration and electroporation． Journal of Microbiological Methods, 2007, 69: 512-517．
　　17． Shan LT, Wang ZL, Ying SH, Feng MG． Hydrophobicity-related protein contents and surface areas of aerial conidia are useful traits for formulation design of fungal biocontrol agents． Mycopathologia, 2010, 169: 483-494．
　　18． Xie XQ, Wang J, Ying SH, Feng MG． A new manganese superoxide dismutase identified from Beauveria bassiana enhances virulence and stress tolerance when overexpressed in the fungal pathogen． Applied Microbiology and Biotechnology, 2010, 86:1543–1553．
　　19． Feng MG, Chen C, Shang SW, Ying SH, Shen ZC and Chen XX． Aphid dispersal flight disseminates fungal pathogens and parasitoids as natural control agents of aphids． Ecological Entomology, 2007, 32: 97-104．
　　20． Zhang GZ, Feng MG, Chen C and Ying SH． Opportunism of Conidiobolus obscurus stems from depression of infection in situ to progeny colonies of host alatae as disseminators of the aphid-pathogenic fungus． Environmental Microbiology, 2007, 9: 859-868．
　　21． Feng MG, Pu XY, Ying SH and Wang YG． Field trials of an oil-based emulsifiable formulation of Beauveria bassiana conidia and low application rates of imidacloprid for control of false-eye leafhopper Empoasca vitis in southern China． Crop Protection, 2004, 23: 489-496．
　　22． Feng MG, Chen B and Ying SH． Trials of Beauveria bassiana, Paecilomyces fumosoroseus and imidacloprid for management of Trialeurodes vaporariorum (Homoptera: Aleyrodidae) on greenhouse grown lettuce． Biocontrol Science and Technology, 2004, 14: 531-544．

　　核心期刊
　　1． 應(yīng)盛華和馮明光． 冠狀耳霉產(chǎn)堿性蛋白酶及其活性． 菌物系統(tǒng), 2000, 19(1): 128-131．
　　2． 應(yīng)盛華和馮明光． 球孢白僵菌孢子乳懸劑的配方研制． 植物保護(hù)學(xué)報(bào)． 2001, 28(4): 345-351．
　　3． 應(yīng)盛華． 球孢白僵菌制劑． 杭州科技, 1999, 6: 18-20．
　　4． 應(yīng)盛華, 徐均煥和周杭松． 冠狀耳霉堿性蛋白酶的生產(chǎn)及應(yīng)用． 杭州科技, 2000, 4: 29-30．
　　5． 應(yīng)盛華和馮明光． 真空干燥球孢白僵菌純孢粉的活孢率、毒力與貯存期． 微生物學(xué)通報(bào)． 2002, 29(5): 42-47．
　　6． 應(yīng)盛華和馮明光． 絲孢類(lèi)蟲(chóng)生生防真菌制劑研制的技術(shù)瓶頸與改良途徑． 中國(guó)蟲(chóng)生真菌研究與應(yīng)用, 北京：中國(guó)農(nóng)業(yè)科學(xué)技術(shù)出版社, 2003, 64-74．
　　7． 應(yīng)盛華, 馮明光和許壽濤． 球孢白僵菌分生孢子乳懸劑對(duì)甘藍(lán)上桃蚜的田間控制效果． 應(yīng)用生態(tài)學(xué)報(bào)． 2003,14(4): 545-548．
　　8． 應(yīng)盛華和馮明光, 許壽濤和馬再波． 球孢白僵菌分生孢子乳懸劑對(duì)甘藍(lán)上桃蚜的田間控制效果． 應(yīng)用生態(tài)學(xué)報(bào)． 2003,14(4): 545-548
　　9． 應(yīng)盛華和馮明光． 不同培養(yǎng)基質(zhì)上球孢白僵菌孢子與菌絲的多糖含量變化?． 菌物學(xué)報(bào)． 2005, 24(2):304-305．
　　10． 應(yīng)盛華, 鄧米霞和馮明光． 三種絲孢類(lèi)生防真菌孢子類(lèi)疏水蛋白的含量與組分比較． 浙江大學(xué)學(xué)報(bào), 2006, 32(4): 401-405．
　　11． 僧明華, 應(yīng)盛華和馮明光． 模擬昆蟲(chóng)中腸環(huán)境對(duì)球孢白僵菌分生孢子的影響． 浙江大學(xué)學(xué)報(bào)(農(nóng)業(yè)與生命科學(xué)版), 2009, 35(3): 261-265．
　　12． 馮明光和應(yīng)盛華． 不同含水量和溫度下貯存中球孢白僵菌分生孢子活力與內(nèi)源營(yíng)養(yǎng)的衰變． 應(yīng)用生態(tài)學(xué)報(bào)． 2002, 13(4): 439-443．
　　13． 楊旭芬, 應(yīng)盛華和馮明光． 限制性?xún)?nèi)切酶介導(dǎo)的玫煙色擬青霉原生質(zhì)轉(zhuǎn)化體系構(gòu)建． 浙江大學(xué)學(xué)報(bào)(農(nóng)業(yè)與生命科學(xué)版), 2006, 32(6): 606-612．
　　14． 秦文彥, 程潔, 應(yīng)盛華和馮明光． 可污染食品及飼料的產(chǎn)黃曲霉毒素真菌的多重PCR檢測(cè)． 菌物學(xué)報(bào), 2007, 26(3): 448-454．
　　15． 許壽濤, 應(yīng)盛華和馮明光． 十種常用農(nóng)藥與球孢白僵菌的生物學(xué)相容性． 植物保護(hù)學(xué)報(bào)． 2002, 29(2): 158-162．
　　16． 許壽濤, 馮明光和應(yīng)盛華． 球孢白僵菌對(duì)桃蚜接種后特定時(shí)間內(nèi)的侵染率． 應(yīng)用生態(tài)學(xué)報(bào)． 2002, 13(6): 701-704．
　　17． 張麗靖, 馮明光和應(yīng)盛華． 球孢白僵菌液體培養(yǎng)條件下淀粉酶的產(chǎn)生及活性影響因子． 微生物學(xué)報(bào), 2002, 42(6): 602-607．
　　18． 頓玉慧, 馮明光和應(yīng)盛華． 新型球孢白僵菌孢子懸乳劑的高效殺蚜活性及其評(píng)價(jià)方法． 微生物學(xué)報(bào), 2003, 43(6): 781-787．

　　會(huì)議論文：
　　1． Ying S-H, Feng M-G & Xu S-T． Field efficacy of emusifiable suspension of Beauveria bassiana conidia for control Myzus persicae population on Chinese cabbage． VIII International Colloquium on Invertebrate 　Pathology and Microbial Control． Foz do Igussu, Brazil, 18-23 August 2002, 64．
　　2． Feng M-G & Ying S-H． Interactive effect of water content and temperature on viability of Beauveria bassiana conidia and metabolism of internally reserved nutrients in storage． VIII International Colloquium on Invertebrate Pathology and Microbial Control． Foz do Igussu, Brazil, 18-23 August 2002, 112-113．
　　3． Xu S-T, Feng M-G & Ying S-H． Time-specific infectionrate of Beauveria bassiana conidia on Myzus persicae under controlled conditions． VIII International Colloquium on Invertebrate Pathology and Microbial Control． Foz do Igussu, Brazil, 18-23 August 2002,102-103．
　　4． Feng M-G & Ying S-H． Development of Beauveria bassiana-based mycoinsecticide for tea leafhopper control in China: Current status and prospects． The 38th annual meeting of Society for Invertebrate Pathology． University of Alaska, Anchorage, USA, Aug． 7-12, 2005, p80．
　　5． Ying S-H & Feng M-G Purification and gene cloning of a new hydrophobin-like protein that relates to thermal tolerance of aerial conidia of fungal biocontrol agents． The 38th annual meeting of Society for Invertebrate Pathology． University of Alaska, Anchorage, USA, Aug． 7-12, 2005, p50．
　　6． Ying S-H & Feng M-G Thiredoxin from the entomopathogenic fungi, Beauveria bassiana: Gene cloning, characterizationand functional expression． The 42nd annual meeting of Society for Invertebrate Pathology． Park city, Utah, USA, Aug． 16-20, 2009, p82．

　　專(zhuān)利成果：
　　1、應(yīng)盛華、馮明光. 一種抗逆能力增強(qiáng)的生防真菌構(gòu)建方法及抗逆能力增強(qiáng)的生防菌株. 公開(kāi)(公告)號(hào) CN101392259, 公開(kāi)(公告)日 2009-03-25.
　　2、馮明光, 應(yīng)盛華. 防治刺吸式口器害蟲(chóng)的菌株及其制劑. 中國(guó)發(fā)明專(zhuān)利號(hào)：ZL 200510060449.8 (2008-7-9)
　　3、 馮明光, 葉素丹, 應(yīng)盛華, 陳春. 2005. 一種高純度孢子粉生產(chǎn)工藝及所用的立式多層產(chǎn)孢箱. 中國(guó)發(fā)明專(zhuān)利號(hào)：ZL 200510050499.8 (授權(quán)日：2008-04-30)

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